Polo studied ballet beginning at age five. After winning a modeling contest, she moved to New York City at the age of seventeen to pursue an acting career.
She has guest starred on many TV shows like The Practice, Felicity, Chicago Hope, Sports Night, and Frasier. She became a regular cast member on the last season of Northern Exposure, and the sixth and seventh seasons of The West Wing as Helen Santos, the wife of Democratic presidential candidate Matt Santos, played by Jimmy Smits. She has also hosted several ceremonies.
Polo has confirmed that she is signed on to reprise her role of Pamela Byrnes Focker in the upcoming sequel Meet the Little Focker, which is now in pre-production.
She was ranked #42 on the Maxim Hot 100 Women of 2002.
In February 2005 Polo appeared on the cover of, and posed nude in Playboy. The appearance was linked to the release of Meet the Fockers, with cover text that made similar plays on words such as “She's one hot Focker”. Some of her fans expressed regret that she made the appearance
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