Charlotte Ross was discovered in a theatre performance at the age of 13 in her hometown of Winnetka, Illinois. At New Trier High School, she continued her study in performing arts and trained at the Steppenwolf Theater and Second City in Chicago. After moving to Los Angeles, she performed at the Pasadena Playhouse and the Tiffany Theatre.

Ross starred for two seasons in the critically acclaimed cable series Beggars and Choosers. She also starred in the family drama Trinity, created by John Wells, and the mini-series A Will of Their Own. She has had lead roles in television series, including The Five Mrs. Buchanans, The Heights (for which she earned a gold record for her performance of the series theme song) and the telefilms A Kiss So Deadly, Fall Into Darkness, She Says She's Innocent and Dark Waters. Ross has also had many notable guest-starring roles, having portrayed a manic-depressive on Birdland, a strung-out drug addict on ER, and, most recently, Kelsey Grammer's love interest on Frasier.

Her film credits include Touch and Go with Michael Keaton, Love and a .45, Savage Land, Foreign Student and Donuts. She earned two Daytime Emmy Award nominations for her role as Eve Donovan on Days of Our Lives.

Ross is also a singer, having studied opera for five years. She has love and compassion for animals and has collaborated with Jane Goodall Institute, SPCA, and PETA for many years. While on location in Puerto Rico, she adopted a homeless dog named Taco who now befriends her yellow lab, Alex, and her one-eyed Pekinese, Katy. She resides in Los Angeles.